pLink-SS

pLink-SS identifies disulfide-linked peptides from high-throughput mass spectrometry data to map native disulfide bonds and analyze disulfide proteomes.

Key Features:

• High-Throughput Capability: Handles large-scale proteomic analyses using high-throughput mass spectrometry data.
• Precision Identification: Accurately identifies disulfide-linked peptides and maps native disulfide bonds within proteins.
• Comprehensive Mapping: Has been applied to map all native disulfide bonds in a monoclonal antibody and ten standard proteins.
• Disulfide Proteome Analysis: Enabled identification of 199 disulfide bonds in Escherichia coli and 568 disulfide bonds in proteins secreted by human endothelial cells.
• Discovery of Regulatory Disulfides: Facilitates discovery of regulatory disulfide bonds involving catalytic or metal-binding cysteine residues.

Scientific Applications:

• Protein Structure Analysis: Mapping disulfide bonds to inform protein folding and stability studies.
• Functional Proteomics: Identification of regulatory disulfides involving catalytic or metal-binding cysteines to elucidate protein function dynamics.
• Biotechnological Applications: Precise disulfide mapping for development and optimization of therapeutic antibodies and other protein-based drugs.

Methodology:

pLink-SS uses advanced algorithms to process high-resolution mass spectrometric data to identify disulfide-linked peptides.