plotXpress

plotXpress normalizes luminescence data from dual luciferase assays and computes summary statistics and fold changes to support comparative gene expression analysis.

Key Features:

• Data upload and normalization: Import raw luminescence data and correct for sample variation using an internal control for normalization.
• Calculation of averages and fold changes: Compute average expression levels per experimental condition and calculate fold changes relative to a user-selected reference condition.
• Visualization with statistical summaries: Generate dot plots accompanied by statistical summaries with customizable presentation parameters.

Scientific Applications:

• Dual luciferase assay analysis: Standardize and quantify luciferase reporter data from dual luciferase assays.
• Gene expression dynamics: Compare expression levels across experimental conditions to assess gene-expression changes.
• Transcription factor activity studies: Quantify reporter activity to evaluate transcription factor effects on target promoters.
• Promoter/enhancer analyses and regulatory element screening: Assess promoter or enhancer activity and screen regulatory elements using normalized reporter measurements.

Methodology:

Import raw luminescence data, normalize using an internal control, calculate condition means, compute fold changes relative to a selected reference, and generate dot plots with statistical summaries.