PmiRtarbase

PmiRtarbase curates experimentally validated positive microRNA (miRNA)-target interactions to provide a focused repository of cases where ~22-nucleotide miRNAs enhance target gene expression.

Key Features:

• Curated Dataset: 312 curated positive miRNA-target associations involving 119 miRNAs and 169 genes across eight species, derived from 130 published studies.
• Positive Regulation Focus: Entries specifically represent interactions in which miRNAs enhance target gene expression rather than repress it.
• Experimental Annotations: Each entry includes detailed descriptions of the interaction and the specific experimental conditions under which positive regulation was observed.

Scientific Applications:

• Study of miRNA-mediated gene regulation: Enables analysis of mechanisms by which miRNAs upregulate gene expression.
• Comparative and cross-species analyses: The multi-species dataset supports comparative studies of positive miRNA-target interactions.
• Therapeutic research: Supports investigation into miRNA-based therapeutic strategies and exploration of novel regulatory pathways involving positive regulation.

Methodology:

Manual literature curation of experimentally validated positive miRNA-target interactions extracted from 130 published studies across eight species.