PoDCall

PoDCall automates calling and quantification of DNA methylation from droplet digital PCR (ddPCR) data as an R package for standardized epigenetic analysis.

Key Features:

• R package implementation: Implemented as an R package for computational processing of ddPCR methylation data.
• Automated Positive Droplet Calling: Automates identification of positive droplets from ddPCR to enable accurate quantification.
• Quantification and Normalization: Performs quantification of methylation levels and normalization across samples.
• Standardization and Reproducibility: Provides automated, standardized processing to improve reproducibility and comparability of ddPCR methylation results.

Scientific Applications:

• Epigenetic studies: Enables standardized quantification of DNA methylation patterns in epigenetics research.
• Cancer research: Supports detection and quantification of methylation biomarkers in cancer using ddPCR.
• Developmental biology: Facilitates assessment of methylation changes during development.
• Disease progression studies: Allows tracking of methylation alterations associated with disease progression.

Methodology:

Applies computational algorithms and robust statistical approaches for calling positive droplets and normalizing methylation levels from ddPCR data within an R package.