Poji

Poji analyzes podosomes, invadopodia, and other micron-sized punctate structures in fluorescence microscopy images using Fiji (ImageJ) to quantify area, fluorescence intensity, relative enrichment of associated proteins, and radial intensity profiles.

Key Features:

• Podosome Characterization: Quantifies podosome area, fluorescence intensity, relative enrichment of associated proteins, and radial intensity profiles.
• Adaptability: Applicable to invadopodia and other micron-sized punctate structures involved in extracellular matrix degradation.
• Detection Sensitivity: Detects subtle differences in podosomal properties arising from experimental treatments or natural turnover processes.
• Implementation: Implemented as a macro for Fiji (ImageJ) to perform image-based analysis.

Scientific Applications:

• Cellular adhesion and invasion: Characterizes podosome regulation, architecture, and function in studies of cellular adhesion and invasion.
• Protein enrichment and structural analysis: Enables quantification of associated protein enrichment and structural properties relevant to extracellular matrix interactions.
• Cell motility, tissue remodeling, and cancer metastasis: Supports investigations into mechanisms of cell motility, tissue remodeling, and cancer metastasis through analysis of podosome and invadopodia properties.

Methodology:

Implemented as a Fiji (ImageJ) macro that detects and quantifies podosome area, fluorescence intensity, relative protein enrichment, and radial intensity profiles.