PrecisePrimer

PrecisePrimer designs PCR primers for preparation, cloning, and DNA library shuffling by performing batch primer design with polymerase buffer presets and validated melting temperature algorithms.

Key Features:

• Batch Primer Design: Enables batch design of primer libraries for large-scale projects such as DNA library generation and DNA shuffling.
• Advanced Melting Temperature Algorithms: Implements validated melting temperature algorithms that have been cross-validated with other computational tools and experimental data.
• PCR Polymerase Buffer Options: Includes popular PCR polymerase buffers as pre-set options to support primer library generation under different experimental conditions.

Scientific Applications:

• Yeast gene extraction and cloning: Generated a primer library for extraction and cloning of 61 genes from yeast genomic DNA, where default parameters produced primer pairs that amplified targets without PCR condition optimization.

Methodology:

Uses validated melting temperature algorithms cross-validated with other computational tools and experimental data, supports batch primer design, and incorporates pre-set PCR polymerase buffer options.