PREX

PREX classifies peroxiredoxin (Prx/PRDX) cysteine-dependent peroxidase sequences—3,516 entries—into six subfamilies to support studies of antioxidant defense and cell signaling in eukaryotes.

Key Features:

• Subfamily Classification: Uses the Deacon Active Site Profiler (DASP) to identify and classify Prx proteins based on functionally relevant sequence fragments surrounding key residues required for activity.
• Search Parameters: Supports retrieval by protein annotation, accession number, PDB ID, organism name, and protein sequence.
• Output Annotations: Provides subfamily designation, accession and GI numbers, genus and species, and the functional site signature used for classification.
• Sequence Alignment: Presents query sequences aligned with a select group of Prxs to facilitate manual evaluation of sequence similarity.
• Subfamily Synopses: Supplies synopses describing each subfamily's characteristics accompanied by pertinent literature references.

Scientific Applications:

• Oxidative Stress Research: Supports investigation of Prx roles in antioxidant defense mechanisms.
• Cell Signaling Analysis: Enables study of Prx involvement in regulation of cell signaling pathways.
• Disease Mechanism Investigation: Aids research into disease mechanisms associated with Prx function or dysfunction.
• Evolutionary and Functional Diversification: Facilitates elucidation of evolutionary relationships and functional diversification within the Prx enzyme family.

Methodology:

Classification is performed with the Deacon Active Site Profiler (DASP) by identifying functionally relevant sequence fragments (functional site signatures) surrounding key residues and assigning sequences to six subfamilies, and query sequences are aligned against selected Prxs for evaluation.