Primer3Plus

Primer3Plus provides enhanced primer design capabilities by leveraging the Primer3 primer design program to generate primers for cloning and step-wise sequencing.

Key Features:

• Primer3 integration: Uses the Primer3 primer design program to perform primer and oligonucleotide selection.
• Automated primer design: Provides automated workflows for designing primers for cloning and step-wise sequencing.
• Local storage: Stores primer sequences and design settings locally for reuse.
• Input format support: Accepts FASTA and other common sequence formats as input.

Scientific Applications:

• Cloning: Design primers and oligonucleotides to support cloning experiments.
• Step-wise sequencing: Design primers to support step-wise sequencing strategies.

Methodology:

Uses the Primer3 primer design program and accepts FASTA and other common sequence formats as input.