PrimerBank

PrimerBank provides primer sequences for PCR and quantitative PCR (QPCR) in human and mouse to support gene expression analysis, encompassing over 306,800 primer pairs that cover the majority of known genes.

Key Features:

• Comprehensive coverage: Over 306,800 primer pairs covering the majority of known human and mouse genes, with at least one primer pair per gene and multiple alternative pairs available.
• Standardized PCR conditions: Uniform PCR conditions specified across primers to support consistent QPCR workflows.
• Indexed identifiers: Primer entries indexed by GenBank accession numbers, NCBI protein accession numbers, NCBI gene IDs, PrimerBank IDs, NCBI gene symbols, and descriptive keywords.
• Primer pair metadata: Each primer pair includes melting temperature (Tm), specific transcript location, and amplicon size.
• Experimental validation: 26,855 mouse primer pairs were experimentally tested using QPCR, agarose gel analysis, sequencing, and BLAST verification.

Scientific Applications:

• Gene expression quantification: Use in PCR and quantitative PCR (QPCR) assays to measure transcript abundance in human and mouse samples.
• High-throughput QPCR workflows: Standardized PCR conditions facilitate large-scale or high-throughput QPCR experiments.
• Primer selection for transcript-specific amplification: Metadata and BLAST-verified sequences support selection of primers with defined Tm, transcript location, and amplicon size for accurate target amplification.

Methodology:

BLAST verification of primer sequences was performed.