PrimerSeq

PrimerSeq designs RT-PCR primers for validation and quantitative analysis of alternative splicing (AS) events using RNA-seq-derived transcriptome profiles.

Key Features:

• Integration with RNA-Seq data: Incorporates user-provided transcriptome profiles derived from RNA-seq experiments into the primer design process.
• AS-targeted primer design: Designs primers that specifically target alternative splicing (AS) events to enable RT-PCR validation and large-scale quantitative analysis of exon splicing levels.

Scientific Applications:

• RT-PCR validation of AS events: Enables design of primers for RT-PCR validation of alternative splicing events discovered by RNA-seq, supporting quantification of exon splicing levels.
• Large-scale splicing studies: Supports large-scale quantitative studies to quantify and validate AS events, contributing to investigation of gene regulation and implications in health and disease.

Methodology:

Incorporates user-provided transcriptome profiles from RNA-seq into its primer design process to target alternative splicing (AS) events for RT-PCR validation.