ProbioMinServer

ProbioMinServer evaluates the safety and functional potential of probiotic strains by integrating genomic analyses to support assessment for food and healthcare applications.

Key Features:

• Safety Assessment: Evaluates antimicrobial resistance profiles, virulence factors, pathogenicity indicators, and genetic elements including plasmid and prophage genes.
• Functionality Analysis: Analyzes gene clusters related to carbohydrate-active enzymes and metabolites and categorizes probiotic functionality into classes relevant to food and feed applications.
• Genomic Integration: Incorporates next-generation sequencing technologies to investigate the genetic makeup of probiotic strains.
• Database Search and Analysis: Performs database searches on whole-genome sequences in FASTA format from 25 common probiotic species and conducts core-genome multilocus sequence typing to identify phylogenetically related strains.

Scientific Applications:

• Microbiology: Supports characterization and comparison of probiotic strains by combined safety and functional profiling.
• Genomics: Enables core-genome multilocus sequence typing and genome-based analyses to assess genetic relationships among strains.
• Food Science: Informs selection of strains for functional foods and feed through profiling of carbohydrate-active enzymes and metabolite-related gene clusters.
• Healthcare: Aids evaluation of probiotic strains for therapeutic or supplement applications by screening for antimicrobial resistance, virulence, and pathogenicity indicators.

Methodology:

Integrates safety assessments with functionality profiling using next-generation sequencing technologies, database searches of whole-genome FASTA for 25 species, and core-genome multilocus sequence typing.