proglycprot (Prokaryotic Glycoproteins)

proglycprot curates experimentally characterized eubacterial and archaeal glycoproteins and their glycosites to support analysis of prokaryotic glycosylation.

Key Features:

• Extensive Data Repository: Contains 340 unique entries comprising 340 glycoproteins and 334 experimentally validated glycosites.
• ProCGP: Main data section with 95 entries providing detailed characterization of glycosites.
• ProUGP: Supplementary section with 245 entries where glycosylation is confirmed but specific glycosites are uncharacterized.
• Detailed Cross-Referencing: Each entry lists source organism, coding gene, protein details, glycosite specifics, type of glycosylation, attached glycans, associated oligosaccharyltransferases (OSTs) or glycosyltransferases (GTs), and supporting references.
• Unique Entries vs Swiss-Prot: Highlights 174 entries not annotated in the Swiss-Prot release of July 2011.
• Structural Insights: Provides a structure gallery with homology models and available crystal structures of characterized glycoproteins.
• Sequon Analysis Tools: Includes two tools for analysis of prokaryotic sequons, the conserved amino acid sequences surrounding glycosites that differ from typical eukaryotic sequons.

Scientific Applications:

• Comparative Glycobiology: Enables comparative studies of prokaryotic and eukaryotic glycosylation mechanisms using curated glycosite data.
• Therapeutic Target Identification: Supports identification of novel glycoproteins for therapeutic targeting in bacteria and archaea.
• Virulence and Immune Evasion Studies: Facilitates exploration of the role of glycosylation in bacterial virulence and immune evasion.
• Structure-Guided Analysis: Supports structure-guided investigations using homology models and crystal structures.

Methodology:

Homology modeling and inclusion of available crystal structures were used to provide structural data.