pRolocGUI

pRolocGUI provides visualization and exploration of spatial (organelle) proteomics data to support assignment and analysis of protein subcellular localization.

Key Features:

• Integration with pRoloc, pRolocdata, and Shiny: Leverages pRoloc and pRolocdata workflows and Shiny for analysis and interactive visualization of spatial proteomics datasets.
• Interactive visualization: Generates interactive visualizations to examine protein localization across organelles and sub-organellar compartments.
• Support for hyperLOPIT-derived datasets: Accepts data from hyperLOPIT workflows, including extensive fractionation and quantitative high-resolution accurate mass spectrometry.
• Multivariate data analysis: Implements multivariate data analysis methods for assignment of proteins to subcellular locations.

Scientific Applications:

• Organelle organization: Visualizes and analyzes the spatial arrangement of organelles and sub-organellar compartments.
• Protein complexes and functional networks: Aids identification and mapping of protein complexes and functional interaction networks within cells.
• Steady-state dynamics: Supports study of protein distribution and dynamics under steady-state conditions.
• Post-transcriptional and post-translation modifications: Enables investigation of how post-transcriptional and post-translation modifications correlate with protein localization.
• Cellular perturbation studies: Facilitates examination of proteome-level localization changes in response to cellular perturbations.

Methodology:

Uses pRoloc and pRolocdata workflows together with multivariate data analysis for assignment of proteins to subcellular locations.