ProPortal

ProPortal hosts curated genomic, transcriptomic, and metagenomic datasets for Prochlorococcus, Synechococcus, and their cyanophages to enable comparative genomics and ecological analysis.

Key Features:

• Genomic Data: Hosts complete genomes of 13 Prochlorococcus strains, 11 Synechococcus strains, and 28 cyanophage strains that infect these groups for comparative analyses across ecotypes and phages.
• Orthologous Group Clustering: Clusters genes from cyanobacterial and cyanophage genomes into orthologous groups to identify shared and lineage-specific gene families.
• Transcriptomic Insights: Provides transcriptome data for Prochlorococcus ecotypes MED4 and MIT9313, including genes upregulated or downregulated in response to environmental stressors and expression patterns from synchronized 24-hour light-dark cycles.
• Metagenomic Sequences: Archives metagenomic sequences from the Global Ocean Survey encompassing Prochlorococcus, Synechococcus, and phage genomes to assess genetic diversity and population differences across marine habitats.
• Field Data Integration: Includes field data exemplifying cyanobacterial populations in natural environments to contextualize genomic and transcriptomic findings.

Scientific Applications:

• Marine microbiology: Enables analysis of genetic diversity and ecological roles of Prochlorococcus, Synechococcus, and their cyanophages.
• Microbial ecology and population dynamics: Supports linking genomic and metagenomic data with environmental conditions and population differences across marine habitats.
• Evolutionary biology and comparative genomics: Facilitates orthologous group comparisons across ecotypes and phages to study adaptation and evolutionary relationships.
• Gene expression and physiological responses: Enables examination of transcriptome changes, including up- and down-regulation under environmental stressors and diel cycles in MED4 and MIT9313.

Methodology:

Genes from cyanobacterial and cyanophage genomes are clustered into orthologous groups, and genomic, transcriptomic, and Global Ocean Survey metagenomic sequences are archived.