ProSAT+

ProSAT+ maps protein sequence annotations onto three-dimensional structures by integrating UniProt, RCSB Protein Data Bank (PDB), and PDBe SIFTS data and visualizing them with JSmol.

Key Features:

• Database integration: Integrates sequence and structural data from the UniProt KnowledgeBase, the RCSB Protein Data Bank (PDB), and PDBe SIFTS.
• Structure-annotation mapping: Maps sequence annotations to corresponding structural residues to relate sequence features with structural properties.
• Visualization with JSmol: Uses JSmol for interactive three-dimensional visualization of protein structures alongside mapped annotations.
• User-defined annotations via URL parameters: Accepts user-provided sequence annotations through URL parameters for overlay onto structures.

Scientific Applications:

• Functional annotation mapping: Associates sequence features such as domains and post-translational modifications with their structural context.
• Disease mechanism analysis: Aids interpretation of structural anomalies and sequence variants in the context of disease mechanisms.
• Therapeutic design: Informs structure-based design and hypothesis generation for therapeutic interventions.
• Active site identification: Facilitates localization and analysis of catalytic residues and binding sites within structures.
• Evolutionary and comparative analysis: Supports exploration of evolutionary relationships among protein families by mapping conserved sequence features onto structures.

Methodology:

Extracts data from UniProt, RCSB PDB, and PDBe SIFTS; maps annotations to residues and renders interactive 3D visualizations using JSmol; accepts user-defined annotations via URL parameters.