ProSAT2

ProSAT2 visualizes sequence-based, residue-specific annotations on three-dimensional (3D) protein structures to map functional sites and relate mutations to structural context.

Key Features:

• Automated Mapping: Maps non-structure-based functional annotations onto 3D protein structures using data from SwissProt and PROSITE to identify active and functional sites.
• Integration with Databases: Incorporates variant and mutation data from UniProt KnowledgeBase and BRENDA for mapping genetic variations onto structures.
• User-Defined Annotations: Supports custom, user-provided residue-specific annotations for mapping onto protein structures.
• WebMol-based Visualization: Uses the WebMol Java molecular viewer to highlight and group residues and display descriptive text based on criteria such as mutations affecting enzyme activity.
• Large-scale Functional-site Identification: Enables rapid visual identification of functional sites in large protein structure datasets by integrating diverse annotations to study enzyme activities and mutations.

Scientific Applications:

• Functional-site identification: Rapid visual identification of active and functional sites within protein structures.
• Variant and mutation analysis: Mapping variants from UniProt KnowledgeBase and BRENDA onto structures to assess impacts on enzyme activity and protein function.
• Integrative structural-functional analysis: Combining annotations from SwissProt, PROSITE, UniProt KnowledgeBase, and BRENDA to analyze structural-functional relationships in proteins.

Methodology:

Automated mapping of non-structure-based annotations onto 3D structures using SwissProt and PROSITE; incorporation of variant and mutation data from UniProt KnowledgeBase and BRENDA; visualization and residue highlighting via the WebMol Java molecular viewer.