ProSight Lite

ProSight Lite applies fixed and variable modifications to amino acid sequences, including glycosylations, to enable iterative hypothesis testing of post-translational modification presence and location in top-down proteomics.

Key Features:

• Modification Matching: Applies fixed and variable modifications to amino acid sequences, including glycosylations, to model post-translational modifications.
• Iterative Hypothesis Testing: Supports iterative evaluation of candidate PTM presence and site localization.
• Scoring Systems: Uses comprehensive scoring systems to assess and rank modification hypotheses.
• Fragmentation Maps: Produces detailed fragmentation maps for localization of PTMs on intact proteins.

Scientific Applications:

• Top-Down Proteomics: Facilitates identification and localization of PTMs in intact proteins analyzed directly by mass spectrometry to study protein function, regulation, and interaction networks.

Methodology:

Applies fixed and variable amino acid modifications (including glycosylations), performs iterative hypothesis testing of PTM presence and location, evaluates hypotheses with comprehensive scoring systems, and generates detailed fragmentation maps.