ProSplicer

ProSplicer catalogs putative alternative splicing events in human genes by integrating alignments of proteins, mRNA sequences, and expressed sequence tags (ESTs) to human genomic DNA to provide evidence for splice variants.

Key Features:

• Database of human splicing events: Stores putative alternative splicing events and associated alignment evidence for human genes.
• Alignment-based evidence compilation: Compiles alternative splicing information from comprehensive alignments of proteins, mRNA sequences, and ESTs to human genomic DNA.
• Multi-source integration: Integrates protein, mRNA, and EST-derived signals to strengthen evidence for splice variant identification.
• Annotation of putative splice variants: Annotates predicted splice variants based on alignment-derived features.

Scientific Applications:

• Alternative splicing discovery: Identify and catalog putative alternative splicing events across human genes using multi-source alignment evidence.
• Splice variant validation and annotation: Provide alignment-based evidence to support and annotate predicted splice variants.
• Tissue-specific expression studies: Investigate implications of alternative splicing on tissue-specific gene expression patterns.
• Gene regulation and transcript diversity analysis: Study how alternative splicing contributes to gene regulation and transcript diversity.

Methodology:

Compile putative alternative splicing information by aligning proteins, mRNA sequences, and expressed sequence tags (ESTs) to human genomic DNA and integrating the resulting alignment-derived signals.