PROSS

PROSS optimizes protein sequences to increase stability and heterologous expression by applying automated structure- and sequence-based design.

Key Features:

• Automated design methodology: Employs an automated structure- and sequence-based design approach to predict mutations that enhance protein stability and expression.
• Structural and sequence integration: Integrates protein structural data and sequence analysis to evaluate mutation effects and preserve function while improving robustness.
• PROSS2 sequence-analysis improvements: Implements more precise sequence analysis techniques for design calculations.
• PROSS2 quality control: Includes improved error handling and an automated assessment of sequence alignment quality used in design.

Scientific Applications:

• Protein Engineering: Designing stability-enhanced variants for industrial and therapeutic protein development.
• Structural Biology: Producing more stable proteins for structural characterization and biophysical studies.
• Biotechnology: Generating robust proteins suitable for biotechnological processes and applications.

Methodology:

Performs comprehensive analysis of protein structures and sequences and integrates that data to predict modifications that enhance stability without compromising function; PROSS2 adds sequence-alignment quality checks and improved error handling.