Proteoform Suite

Proteoform Suite identifies and quantifies intact proteoforms from MS1 data by comparing experimental masses to a database of known protein sequences to detect post-translational modifications and amino acid variations.

Key Features:

• Direct MS1 Identification: Identifies proteoforms directly from MS1 data by measuring intact masses and mass differences indicative of post-translational modifications and amino acid variations.
• Quantification: Quantifies intact proteoform abundances using MS1 signal intensities.
• Database Comparison: Compares experimental proteoform masses against a database of known protein sequences for assignment of identities.
• No MS/MS Required: Enables identification and quantification without requiring MS/MS fragmentation.
• Direct Mass Analysis: Employs direct mass analysis to enhance sensitivity and accuracy in proteomic studies.

Scientific Applications:

• Gene-Level Analysis: Visualizes and analyzes proteoform families derived from individual genes, providing a comprehensive view of proteoforms associated with specific genetic sequences.

Methodology:

Direct mass analysis of MS1 data with comparison of experimental proteoform masses to a database of known protein sequences; identification and quantification are based on measured mass differences indicative of post-translational modifications and amino acid variants and do not require MS/MS fragmentation.