PROWL

PROWL identifies proteins from mass spectrometry data by analyzing MS/MS spectra and peptide mass fingerprints to support proteomic analyses.

Key Features:

• PepFrag: Analyzes single spectra from tandem mass spectrometry (MS/MS) to deconvolute spectra and identify peptide sequences.
• GPM (General Peptide Mass): Processes multiple MS/MS spectra simultaneously to enable high-throughput peptide identification for large-scale proteomic studies.
• ProFound: Analyzes peptide mass fingerprinting (PMF) single-spectrum data to identify proteins based on peptide mass fingerprints.

Scientific Applications:

• Protein identification from complex samples: Identifies proteins using MS/MS and peptide mass fingerprinting data from biological samples.
• High-throughput proteomics: Enables simultaneous processing of multiple MS/MS spectra for large-scale peptide and protein identification studies.
• Peptide mass fingerprinting studies: Supports single-spectrum PMF experiments for protein identification based on mass signatures.

Methodology:

Integration of computational components (PepFrag, GPM, ProFound) that accept raw mass spectrometer data and process spectra to extract peptide and protein sequence information.