PSSA

PSSA categorizes CHIKV sequences into geographical genotypes using phylogenetic analysis to characterize sequence variation and inform studies of viral spread and evolution.

Key Features:

• Phylogenetic Analysis: Assigns CHIKV sequences to West African (WAf), East/Central/South African (ECSA), and Asian genotypes using phylogenetic methods.
• Nucleotide/Amino Acid Sequence Analysis: Analyzes nucleotide and amino acid variation from conventional Sanger sequencing data to identify amino acid substitutions.
• Data Visualization: Produces visual representations of sequence variation and genotype assignments to aid interpretation.

Scientific Applications:

• Virology/Public Health Surveillance: Enables rapid identification of CHIKV genotypes to support surveillance and timely interventions during outbreaks.

Methodology:

Phylogenetic analysis of CHIKV sequences and nucleotide/amino acid sequence analysis using conventional Sanger sequencing data to detect amino acid substitutions.