pycofitness

pycofitness performs in silico mutagenesis of protein and RNA sequences using coevolutionary analysis to predict the impact of mutations.

Key Features:

• Coevolutionary analysis: Predicts mutation effects using direct coupling analysis by pseudo-likelihood maximization.
• Sequence mutagenesis: Supports in silico mutagenesis of both protein and RNA sequences.
• Efficient implementation: Provides computationally efficient prediction of mutation effects.

Scientific Applications:

• Genetic variant deleteriousness prediction: Predicts the deleteriousness of genetic variants.
• Protein fitness prediction: Predicts the effect of mutations on protein fitness.
• Thermodynamic stability prediction: Predicts the effect of mutations on protein thermodynamic stability.

Methodology:

Direct coupling analysis by pseudo-likelihood maximization.