pyFuRNAce

pyFuRNAce integrates the entire RNA origami workflow from motif definition and blueprint design to sequence generation and primer selection into a single platform for designing complex RNA nanostructures, with particular focus on co-transcriptional RNA origami.

Key Features:

• Motif-based assembly paradigm: Enables users to create and modify custom RNA nanostructures through a motif-based assembly approach.
• Blueprint design: Supports the creation of structural blueprints for RNA origami designs.
• Sequence generation: Generates nucleotide sequences for designed RNA nanostructures.
• Primer selection: Includes automated primer selection for the generated sequences.
• Real-time 3D visualization: Provides real-time three-dimensional visualization of RNA nanostructures during the design process.
• Co-transcriptional design focus: Specialized for co-transcriptional RNA origami, enabling the design of structures that fold during transcription.

Scientific Applications:

• Self-assembling RNA filaments: Used to design self-assembling RNA filament structures.
• RNA droplets: Used to design RNA droplet structures.
• Large co-transcriptional RNA origami: Used to design large co-transcriptional RNA origami structures, including one consisting of 2501 nucleotides validated as the largest co-transcriptional RNA origami to date.
• RNA nanotechnology: Accelerates the development of functional RNA origami structures for applications in medicine, biotechnology, and synthetic biology.

Methodology:

pyFuRNAce is built around a motif-based assembly paradigm and integrates the RNA origami workflow from motif definition through blueprint design to sequence generation and primer selection.