Pyxis

Pyxis supports untargeted and targeted analysis workflows for ion mobility spectrometry imaging (IMS-MSI) data, enabling analysis of complex multidimensional datasets from intact tissue sections.

Key Features:

• Vendor-neutral data analysis: Pyxis processes IMS-MSI data independent of instrument manufacturer, enabling broad accessibility across different mass spectrometry imaging platforms.
• Untargeted analysis workflow: Pyxis enables discovery-based analysis of complex IM-MSI datasets without prior knowledge of target analytes.
• Targeted analysis workflow: Pyxis supports focused analysis of specific known analytes in IM-MSI datasets.
• Isobaric species resolution: Pyxis leverages ion mobility separation to distinguish isobaric species that cannot be resolved by mass alone.

Scientific Applications:

• Metabolomic spatial analysis: Pyxis enables metabolite imaging and functional assignment directly from intact tissue sections.
• Mouse kidney tissue analysis: Pyxis has been demonstrated for IMS-MSI data analysis of mouse kidney tissue.
• THP-1 monocyte analysis: Pyxis has been demonstrated for IMS-MSI data analysis of THP-1 monocytes.

Methodology:

Pyxis employs ion mobility spectrometry as an orthogonal separation dimension to supplement the lack of chromatographic separation in mass spectrometry imaging, enabling multidimensional data analysis workflows.