Q4SRM

Q4SRM evaluates signal quality of stable-isotope heavy-labeled peptide standards in LC-SRM-MS targeted proteomics experiments to support quality control of SRM-based quantification.

Key Features:

• Heavy-labeled peptide assessment: Rapidly assesses signal quality from all stable-isotope heavy-labeled peptides within a sample.
• Four QC metrics: Employs four distinct metrics to quantify signal quality for SRM data.
• Dual-level analysis: Evaluates both individual SRM transitions and the collective group of transitions associated with a single peptide.
• Automated flagging: Flags transitions or peptide groups that do not meet predefined quality-control metrics.
• High-throughput compatibility: Designed to handle SRM experiments monitoring hundreds to thousands of peptides across samples.

Scientific Applications:

• Targeted proteomics QC: Quality control for selected reaction monitoring (SRM) workflows in targeted proteomics.
• Standard verification: Verification of stable-isotope heavy-labeled peptide standards used for endogenous peptide quantification.
• Quantitative accuracy assurance: Ensures reliability of SRM-based quantification for clinical biomarker measurement and cellular modeling studies.
• Post-acquisition QC: Applied immediately after liquid chromatography-SRM-MS (LC-SRM-MS) analysis for dataset quality assessment.

Methodology:

Computes four distinct metrics applied to individual SRM transitions and to grouped transitions for each peptide, comparing values against predefined quality-control thresholds and flagging those that fail.