QATS

QATS automates the identification and quantification of toroidal nuclei in biological images, complementing micronucleus analysis for chromosomal instability biomarker assessment.

Key Features:

• Toroidal nucleus identification: QATS automates detection of toroidal nuclei with minimized false positives while maintaining high agreement with manual quantifications.
• Micronucleus identification: QATS identifies micronuclei to enable convenient comparison of both chromosomal instability biomarkers.
• Large-scale assessment: QATS enables assessment of chromosomal instability biomarker frequency for determination of chromosomal instability levels in cellular models.

Scientific Applications:

• Chromosomal instability biomarker quantification: QATS quantifies toroidal nuclei and micronuclei as complementary chromosomal instability biomarkers in cellular models.
• Stress response analysis: QATS facilitates evaluation of biological stresses leading to formation of chromosomal instability-associated phenotypes across different genetic backgrounds and environmental conditions.

Methodology:

QATS is implemented as an ImageJ plugin. The sources do not provide further explicit computational methodological detail.