QCPA

QCPA quantifies cellular biochemical activities in cancer cells by using targeted mass spectrometry to measure abundance and stoichiometry of regulatory post-translational modifications (PTMs) and total protein levels.

Key Features:

• Targeted Mass Spectrometry Assays: Quantifies the abundance and stoichiometry of regulatory PTMs on sentinel proteins.
• Peptide Coverage: Profiles 1,913 peptides derived from 469 effector proteins.
• Isotopically Labeled Triplets: Utilizes triplets of isotopically labeled peptides to cover known post-translational regulatory sites and determine stoichiometries.
• Total Protein Abundance Measurement: Measures unmodified regions to assess total protein abundance and compare modified and unmodified states.
• TrypQuant Control: Incorporates TrypQuant, a synthetic substrate, to control technical variability in sample preparation and mass spectrometric analysis.

Scientific Applications:

• Chemoproteomics: Supports improved chemoproteomic approaches.
• Cell Signaling and Clinical Proteomics: Facilitates comprehensive analysis of cell signaling pathways and enables clinical proteomics for diagnostic evaluation.

Methodology:

Targeted mass spectrometry assays using triplets of isotopically labeled peptides, measurement of unmodified regions for total protein abundance, and the TrypQuant synthetic substrate to control technical variability in sample preparation and mass spectrometric analysis.