QGRS-H Predictor

QGRS-H Predictor: Phylogenetic mapping of conserved G-quadruplex forming sequences

QGRS-H Predictor identifies and analyzes phylogenetically conserved putative Quadruplex forming G-Rich Sequences (QGRS) across semi-globally aligned nucleotide sequences, including mRNAs, non-coding RNAs (ncRNAs), promoters, telomeres, gene flanking regions, 5′- and 3′-untranslated regions (UTRs), and coding sequences (CDS).

Key Features:

• Conserved QGRS Mapping: Detects conserved putative G-quadruplex forming sequences across aligned nucleotide sequences in diverse genomic contexts.
• Phylogenetic Conservation Analysis: Prioritizes conserved QGRS to improve confidence in computational G-quadruplex predictions and reduce experimental validation requirements in large-scale genomics studies.

Scientific Applications:

• Regulatory Element Identification: Identifies conserved cis-regulatory G-quadruplex motifs in RNA sequences involved in post-transcriptional gene expression.
• Disease-Associated G-quadruplex Analysis: Characterizes distribution and composition of G-quadruplex structures implicated in human health and disease.
• Comparative Genomics: Maps homologous G-quadruplex forming sequences to enhance accuracy of genomic analyses.

Methodology:

Applies semi-global sequence alignment to homologous nucleotide sequences and computationally scans for putative G-quadruplex forming G-rich motifs (QGRS), then evaluates phylogenetic conservation across aligned regions to prioritize biologically relevant candidates.