QUADRatlas

QUADRatlas provides an integrated resource for identification, annotation, and analysis of RNA G-quadruplexes (RG4s) and their interactions with RNA-binding proteins (RBPs) across the human transcriptome to support studies of post-transcriptional regulation and disease mechanisms.

Key Features:

• Comprehensive Database: Integrates experimentally-derived and computationally predicted RG4s across the human transcriptome with annotations related to biological functions and disease associations.
• RBP Interaction Data: Contains a curated collection of known interactions between RG4s and RBPs and an extensive dataset of RBP binding sites.
• Hypothesis Generation Tools: Enables intersection of RG4 data with potential regulators and effectors to generate hypotheses about RG4 regulation, function, and pathogenicity.
• Predictive Analysis Tools: Provides prediction of RBP binding to RG4s, assessment of RG4 enrichment within specific gene sets, and de novo RG4 prediction capabilities.

Scientific Applications:

• Post-transcriptional regulation studies: Characterizes RG4 roles as post-transcriptional regulators of gene expression by linking RG4 locations and annotations to regulatory outcomes.
• RBP–RG4 interaction analysis: Supports integrated analysis of RG4s and their interactions with RBPs to map regulatory networks.
• Disease association and therapeutic research: Facilitates identification of disease-associated RG4s and informs development of therapeutic strategies targeting RG4-related pathways.

Methodology:

Integration of experimentally-derived and computationally predicted RG4s, curation of known RG4–RBP interactions and RBP binding sites, predictive analyses for RBP binding to RG4s, RG4 enrichment assessment within gene sets, and de novo RG4 prediction.