QuantifyPolarity

QuantifyPolarity quantifies planar polarized protein distributions and cell morphological properties in developing tissues to measure planar polarization.

Key Features:

• Planar Polarity Quantification: Uses Principal Component Analysis (PCA) to quantify planar polarity in a manner reported to be insensitive to variations in cell geometry and validated across biological datasets and simulated models.
• Automated Cell Shape and Morphology Analysis: Provides image analysis routines to assess cell morphology and packing geometry.
• Multiple Polarity Quantification Methods: Implements three distinct automated methods for polarity quantification.
• Mathematical Quantification and High-throughput Analysis: Includes comprehensive mathematical formulas for polarity metrics and supports high-throughput analysis of protein distributions and cell properties.

Scientific Applications:

• Epithelial Tissue Analysis: Quantifies asymmetric distributions of planar polarity proteins in epithelial tissues to study planar polarization.
• Comparative and Simulation Studies: Enables comparison of protein distribution and cell property metrics across biological datasets and simulated models.

Methodology:

Principal Component Analysis (PCA) for polarity quantification; automated image-based analysis of cell shape, morphology, and packing geometry; use of comprehensive mathematical formulas; validation on biological datasets and simulated models.