QuantProReloaded

QuantProReloaded performs statistical analysis and visualization of microspot immunoassay (MIA) data to enable quantitative proteomics, including protein and phosphoprotein assessment in complex samples.

Key Features:

• Statistical analysis: Performs statistical analyses tailored to microspot immunoassay (MIA) datasets.
• Time-resolved and non-time-resolved assays: Supports analysis modes for both time-resolved and non-time-resolved MIA experiments.
• Data processing: Processes MIA data for downstream quantitative interpretation.
• Data visualization: Generates visualizations for inspection of MIA results and quantitative trends.
• Proteomics focus: Targets quantitative assessment of proteins and phosphoproteins in proteomics workflows.
• Complex sample compatibility: Applicable to analyses of complex biological samples such as cell lysates and blood plasma.
• Implementation: Implemented using R and Java.
• MIA format suitability: Designed for the microspot immunoassay format, which uses limited sample and reagent volumes.

Scientific Applications:

• Quantitative proteomics: Quantifies protein and phosphoprotein abundance from microspot immunoassays for proteomic studies.
• Cell lysate analysis: Enables quantitative analysis of proteins in cell lysate samples.
• Blood plasma analysis: Enables quantitative analysis of proteins in blood plasma samples.
• Biological research and clinical diagnostics: Supports applications in biological research and clinical diagnostic investigations that use MIA-based measurements.

Methodology:

Implemented in R and Java; performs statistical analysis, data processing, and visualization for time-resolved and non-time-resolved microspot immunoassay (MIA) data.