QuantWorm

QuantWorm: Automated High-Throughput Imaging for Caenorhabditis elegans Phenotypic Quantification

QuantWorm enables automated, high-throughput phenotypic analysis of Caenorhabditis elegans by acquiring and processing microscope images and videos to extract quantitative measurements of lifespan, locomotion, body size, and reproduction.

Key Features:

• Data Acquisition Module: Captures high-resolution images and videos using a microscope with digital camera and motorized stage for downstream quantitative analysis.
• WormLifespan: Counts moving worms from two time-lapse images to quantify lifespan-associated survival.
• WormLocomotion: Computes velocity of moving worms to measure locomotor activity and behavior.
• WormLength: Measures worm body length to assess growth and development.
• WormEgg: Quantifies egg counts to evaluate reproductive output.

Scientific Applications:

• Genetic and Drug Assays: Performs high-throughput quantification of multiple C. elegans phenotypes for genetic screening and pharmacological studies.

Methodology:

QuantWorm integrates automated image acquisition with computational image analysis modules to process time-lapse images and videos, detect and segment worms and eggs, and extract quantitative phenotypic metrics for large-scale experimental datasets.