QUAST

QUAST evaluates and compares genome assemblies by computing multiple assembly-quality metrics to assess contiguity, accuracy, and completeness with or without a reference genome.

Key Features:

• Reference-aware and reference-free evaluation: Supports assembly assessment both with a reference genome and in reference-free contexts for unsequenced species.
• Assembly-quality metrics: Computes numerous metrics that capture contiguity, accuracy, and completeness of assemblies.
• Support for finished and draft genomes: Applies metrics and comparisons to both finished and draft genome assemblies.
• Comparative analysis: Enables direct comparison of multiple assemblies and assemblers across datasets.
• Analytical outputs: Produces detailed reports, summary tables, and plots summarizing assembly metrics.

Scientific Applications:

• Assembler benchmarking: Comparative evaluation of different genome assemblers across diverse datasets.
• Quality assessment of assemblies: Quantitative assessment of contiguity, accuracy, and completeness for finished and draft genomes.
• Evaluation of assemblies from unsequenced species: Assessment of assemblies when reference genomes are unavailable.
• Preparation of results for publication: Generation of detailed metric summaries and plots for reporting assembly quality.

Methodology:

Computes a variety of assembly-quality metrics and generates detailed reports, summary tables, and plots for assemblies evaluated with or without a reference genome.