QuoVadoPro

QuoVadoPro quantifies intracellular cargo movement by measuring temporal variance in pixel illumination across time-lapse fluorescence images to infer intracellular motility.

Key Features:

• Autonomous Quantification: Performs automated measurement of fluorescently tagged intracellular cargo movement without manual tracking.
• Temporal Variance Analysis: Analyzes variance in pixel illumination across time-lapse images to infer motility rather than tracking individual particles.
• Versatility Across Experimental Paradigms: Operates across diverse experimental conditions and cell types.
• Applicability to Non-Neuronal Cells: Suited for non-neuronal cells with short, multidirectional cargo movements that challenge kymography and particle tracking.
• Compatibility with Low Resolution Images: Processes images acquired with low temporal or spatial resolution.
• Adaptability to Various Cargo Types: Analyzes intracellular cargo of varying shapes and sizes, including mitochondria and the endoplasmic reticulum.

Scientific Applications:

• Understanding Fundamental Cellular Processes: Quantifies organelle and cargo dynamics to study cellular homeostasis.
• Studying Disease Mechanisms: Investigates disruptions in intracellular trafficking associated with cancer and neurodegenerative disorders.
• Testing Therapeutic Pharmaceuticals: Assesses drug effects on intracellular dynamics for pharmacological studies.

Methodology:

Computes temporal variance of pixel intensities across time-lapse fluorescence images to quantify intracellular motility instead of performing particle tracking or kymography.