racoon_clip

racoon_clip processes iCLIP and eCLIP data to extract RNA binding signal at single-nucleotide resolution.

Key Features:

• Automated Pipeline: Provides fully automated processing of iCLIP and eCLIP data with multiple pre-settings and fully customizable parameters.
• Summary Report Generation: Outputs a conclusive summary report with visualizations and statistics for all analysis steps.
• Workflow Engine: Implemented as a snakemake-powered command line tool using snakemake version >= 7.22 and Python version >= 3.9.

Scientific Applications:

• iCLIP Data Analysis: Analyzes individual-nucleotide resolution UV crosslinking and immunoprecipitation data to identify RNA-protein interaction sites at single-nucleotide resolution.
• eCLIP Data Analysis: Analyzes enhanced CLIP data to extract RNA binding signal with improved signal-to-noise ratio at single-nucleotide resolution.

Methodology:

racoon_clip is implemented as a snakemake-powered pipeline; specific computational steps for data processing are not explicitly detailed in the sources.