RADAR

RADAR annotates and prioritizes genetic variants in the post-transcriptional regulome of RNA-binding proteins (RBPs) to assess their potential functional impact using ENCODE-RBP and integrated genomic features.

Key Features:

• ENCODE-RBP integration: Integrates ENCODE-RBP experimental data for RBP binding and regulome mapping.
• Conservation metrics: Evaluates sequence conservation to inform variant impact.
• RNA structure analysis: Assesses RNA secondary-structure features at variant sites.
• Network centrality metrics: Computes network centrality measures to prioritize variants affecting central RBP-regulatory nodes.
• Motif recognition: Detects RBP-binding motif occurrences overlapping variants.
• Overall impact scoring: Synthesizes multiple feature scores into a single variant impact score.
• Tissue-specific analysis: Incorporates tissue-specific inputs to refine variant relevance.
• Somatic and germline variant identification: Identifies and scores both somatic and germline variants for RBP-function dysregulation.

Scientific Applications:

• Disease research: Pinpoints variants linked to RBP dysfunction to study molecular mechanisms in diseases including cancer.
• Variant prioritization: Ranks variants by predicted post-transcriptional impact to prioritize candidates for functional validation.

Methodology:

Integrates ENCODE-RBP experimental datasets and applies computational analyses of sequence conservation, RNA structural features, network centrality metrics, and RBP-motif occurrences, then combines these features with tissue-specific information to compute an overall impact score per variant.