RAPID

RAPID (Rep-Seq Dataset Analysis Platform with Integrated Antibody Database): Integrated Human IGH Repertoire Analysis Database

RAPID consolidates and analyzes human IGH Rep-seq datasets, integrating therapeutic and antigen-specific antibody sequences to enable comparative repertoire analysis and clone annotation across multiple health conditions.

Key Features:

• Integrated Antibody Database: Aggregates 521 WHO-recognized therapeutic antibodies, 88,059 antigen- or disease-specific antibodies, and 306 million clones from 2,449 human IGH Rep-seq datasets spanning 29 health conditions.
• Standardized Rep-seq Analysis Pipeline: Processes and analyzes user-submitted Rep-seq datasets within a consistent computational framework.
• Comparative Repertoire Analysis: Compares uploaded repertoires against reference datasets to identify shared or unique antibody sequences across conditions.
• Automatic Clone Annotation: Annotates clones using integrated therapeutic and known antibody references.
• Sequence- and Keyword-Based Querying: Supports targeted searches using antibody sequence data or keywords.

Scientific Applications:

• Humoral Immunity Profiling: Characterizes antibody repertoire diversity and clonal composition in adaptive immune studies.
• Antibody Discovery: Identifies candidate therapeutic antibodies through comparison with known antigen- and disease-specific sequences.
• Disease-Associated Repertoire Analysis: Detects repertoire features associated with specific health conditions to inform diagnostic and therapeutic research.

Methodology:

RAPID integrates curated therapeutic and antigen-specific antibody datasets with large-scale human IGH Rep-seq data. Submitted repertoires are processed through a standardized pipeline, followed by clone-level comparison and annotation against reference antibody sequences to enable cross-condition repertoire analysis.