RCARE

RCARE identifies and annotates RNA-DNA difference (RDD) sites to support analysis of post-transcriptional RNA editing and its effects on protein function and disease.

Key Features:

• Identification and Annotation: Distinguishes true RNA editing events from false positives to identify RDD sites.
• Visualization: Provides biological annotations and summary plots for interpretation of RNA editing data.
• Comparative Analysis: Supports comparative analyses across multiple samples to detect condition-specific RDD sites and explore their functional roles.
• Reliability Assessment: Generates evidence levels for identified RDD sites by integrating information from scientific publications and databases.
• Data Compatibility: Accepts sequence-based alignment files that can be converted into VCF format using a Python script for upload and analysis.

Scientific Applications:

• Functional role elucidation: Enables investigation of how RNA editing affects protein function.
• Disease mechanism investigation: Supports analysis of RNA editing contributions to human disease phenotypes.
• Condition-specific editing analysis: Facilitates comparison of RDD site occurrence and annotation across conditions or sample groups.

Methodology:

Leverages thousands of previously known editing sites to enhance search and annotation, integrates evidence from scientific literature and databases to assign evidence levels, and processes sequence-based alignment files that can be converted to VCF via a Python script for analysis.