Re-Annotator

Re-Annotator: Microarray Probe Re-annotation Using Custom mRNA Reference Database

Re-Annotator performs systematic re-annotation of gene expression microarray probe sequences by mapping them to a custom-built mRNA reference database to improve probe-to-gene assignment accuracy.

Key Features:

• Custom mRNA Reference Database: Maps probe sequences to an updated mRNA reference database to refine gene and transcript annotations.
• Microarray Adaptability: Supports re-annotation of gene expression microarrays and can be adapted for other microarray types, including methylation and genotyping arrays.
• Comparative Performance: Demonstrates improved annotation accuracy over ReMOAT in computational evaluations using experimental gene expression data.
• Probe Discrepancy Detection: Identified ~25% probe annotation discrepancies relative to manufacturer annotations on the Illumina Human-HT12 v4 microarray platform.

Scientific Applications:

• High-Throughput Microarray Analysis: Improves reliability of gene expression, methylation, and genotyping studies by providing accurate probe-to-gene mappings.

Methodology:

Re-Annotator cross-references microarray probe sequences against a custom mRNA reference database to remap probes to corresponding genes or transcripts based on current genomic sequence information.