readhit

Browser-based viewer that maps sequencing reads onto one short reference — an amplicon, gene or plasmid. Reads open as Sanger AB1/ABIF, SCF, FASTA, FASTQ or a SAM somebody else already mapped (gzipped files are unpacked in place); the reference as FASTA, GenBank or a read. Both read orientations are tried automatically. The pileup reports per-position depth, where reads disagree with the target, and the consensus — phred-weighted for capillary reads, which keep their chromatogram under the letters. An optional protein lane translates target and consensus side by side. Reads are placed by minimap2 compiled to WebAssembly, or by the built-in aligner. One read or the whole alignment saves as FASTA. Runs entirely in the browser — files are never uploaded.