ReCappable-seq

ReCappable-seq maps transcription start sites (TSSs) across all RNA polymerases at single-nucleotide resolution by enzymatically exchanging 5' m^7G caps and 5' triphosphates for a selectable tag.

Key Features:

• Enzymatic Cap Exchange: Replaces 5' m^7G caps and 5' triphosphates of transcripts with a selectable tag to enable capture of capped and triphosphorylated RNAs.
• Single-nucleotide resolution: Defines TSSs with single-nucleotide precision.
• Comprehensive TSS Identification: Detects Pol II TSSs in agreement with CAGE and reveals genome-wide TSSs from Pol III and other non-Pol II transcripts.
• Broad genomic scope: Locates novel TSSs in both nuclear and mitochondrial genomes.
• Concurrent regulatory landscape analysis: Enables simultaneous interrogation of regulatory landscapes for coding and noncoding RNA.
• Epigenetic profile classification: Classifies epigenetic profiles associated with TSSs from Pol II and non-Pol II transcripts.

Scientific Applications:

• Mapping eukaryotic transcription initiation: Provides detailed maps of TSSs across all RNA polymerases for studies of transcription initiation.
• Discovery of non-Pol II TSSs: Enables genome-wide identification of Pol III and other non-Pol II transcription start sites.
• Regulatory mechanism studies: Supports analysis of regulatory mechanisms governing coding and noncoding RNAs.
• Epigenetic investigations: Facilitates study of epigenetic modifications associated with TSSs.

Methodology:

Computational methods are not specified in the provided description.