REDH

REDH catalogs adenosine-to-inosine (A-to-I) RNA editing events across murine and human hematopoietic samples to integrate and characterize the RNA editome in hematopoietic differentiation and malignancy.

Key Features:

• Extensive Data Integration: Consolidates 30,796 A-to-I editing sites from 12 murine adult hematopoietic cell populations and more than 400,000 edited events across 48 human cohorts of malignant hematopoietic samples, including RNA-seq from 29 leukemia patients and 19 healthy donors obtained via NCBI Gene Expression Omnibus (GEO).
• Differentiation Module: Explores RNA editing patterns associated with normal hematopoietic development.
• Disease Module: Identifies and characterizes abnormal editing sites linked to hematologic diseases and malignancies.
• Enrichment Module: Analyzes the genomic distribution and enrichment of editing events across genomic features.
• Knowledge Module: Integrates clinical metadata for human samples to provide physiological and pathological context for editing events.
• Comparative Analysis: Enables comparison of RNA editing profiles between hematologic malignancies and healthy controls to identify disease-specific editing patterns.

Scientific Applications:

• Understanding Hematopoietic Homeostasis: Provides detailed editome data to investigate RNA editing roles in normal hematopoietic processes and cellular homeostasis.
• Identifying Therapeutic Targets: Highlights editing sites altered in pathological conditions to support the identification of potential therapeutic targets in hematologic malignancies.
• Research and Clinical Insights: Supports studies of RNA editing in disease progression and treatment response by linking editome patterns to clinical metadata.

Methodology:

RNA-seq data from 29 leukemia patients and 19 healthy donors (GEO) and from 12 murine hematopoietic cell populations were downloaded, followed by sequence alignment and identification processes to systematically catalog A-to-I editing sites.