RefFinder

RefFinder: Integrated reference gene stability ranking for qPCR normalization

RefFinder integrates geNorm, NormFinder, BestKeeper, and the comparative ΔCt method to evaluate and rank the stability of candidate reference genes for quantitative PCR normalization.

Key Features:

• Integration of Multiple Statistical Algorithms: Combines geNorm, NormFinder, BestKeeper, and the comparative ΔCt method to assess reference gene expression stability using distinct statistical approaches.
• Weighted Stability Rankings: Assigns weights to gene rankings from each algorithm and calculates a geometric mean to generate consolidated stability rankings.
• Flexible Evaluation Options: Enables analysis of overall rankings or results from individual algorithms or selected combinations.

Scientific Applications:

• Reference Gene Validation: Identifies stable reference genes to improve accuracy and reproducibility in quantitative PCR and gene expression analyses.

Methodology:

Aggregates stability metrics from geNorm, NormFinder, BestKeeper, and the comparative ΔCt method, applies weighted ranking to each candidate reference gene, and computes a geometric mean to produce a final stability order for normalization in gene expression studies.