RELION-5

RELION-5 streamlines electron tomography data processing from movie import to atomic model building using an empirical Bayesian approach.

Key Features:

• End-to-end pipeline: Functionality ranges from import of unprocessed movies to automated building of atomic models in final maps.
• Metadata standardisation: Explicit definition of metadata items describes pipeline steps for interoperability with other software tools.
• Interoperability framework: Metadata structure provides a framework for standardisation across electron tomography processing tools.

Scientific Applications:

• Electron cryo-tomography: Structure determination of macromolecular complexes embedded in complex frozen, hydrated environments.
• Subtomogram averaging: Averaging images of multiple instances of molecules localised in noisy three-dimensional tomographic reconstructions to achieve resolution sufficient for de novo atomic modelling.

Methodology:

Empirical Bayesian approach.