resiDB

ResiDB: Sequence Similarity-Based Database Manager for Diagnostic Assay Design

ResiDB automates the creation and curation of custom DNA sequence databases by organizing sequence clusters from diverse biological sources based on sequence similarity to user-defined input sequences.

Key Features:

• Automated Database Construction: Generates custom sequence databases from bacteria, fungi, viruses, protozoa, invertebrates, plants, archaea, environmental samples, and whole genome shotgun sequences.
• Sequence Similarity-Dependent Curation: Identifies and curates sequence clusters using algorithms that group sequences by similarity to input targets, supporting genes with frequent point mutations such as antibiotic resistance genes.

Scientific Applications:

• Diagnostic Assay Development: Supports design of assays targeting sepsis-relevant pathogens and antibiotic resistance mechanisms within the H2020 FAPIC project.
• Pathogen Detection and Antimicrobial Resistance Research: Enables structured management of complex sequence datasets for microbial identification and resistance gene analysis.

Methodology:

ResiDB applies sequence similarity algorithms to cluster and curate DNA sequences according to user-defined targets, generating comprehensive, research-specific databases for diagnostic and antimicrobial resistance studies.