RF-Cloning.org

RF-Cloning.org automates primer design for restriction-free cloning to enable insertion of any DNA sequence into any plasmid without relying on restriction sites.

Key Features:

• Automated Primer Design: Generates primers for restriction-free (RF) cloning to facilitate insertion of DNA fragments into plasmids without using restriction sites.

Scientific Applications:

• Custom Plasmid Design: Supports genetic engineering and cloning workflows when convenient restriction sites are absent by enabling sequence insertion into plasmids.

Methodology:

Automated primer design for RF-cloning and reference to PCR-based techniques built on QuikChange™ mutagenesis principles.