RGEN

RGEN automates CRISPR target selection and mutant verification using RNA-guided nucleases and next-generation sequencing (NGS) to support DNA-free editing workflows in Chlamydomonas reinhardtii.

Key Features:

• CRISPR Target Design: Identifies optimal CRISPR targets for RNA-guided nucleases with high specificity.
• DNA-Free CRISPR Protocol: Enables targeted knock-outs in Chlamydomonas reinhardtii using DNA-free methods with reduced off-target effects.
• NGS Integration: Analyzes mutants for validation of genetic modifications via high-throughput sequencing (NGS).

Scientific Applications:

• Gene Knock-Out Studies: Facilitates functional analysis in Chlamydomonas reinhardtii for photosynthesis and biofuel research.

Methodology:

Covers the CRISPR workflow from target selection to mutant analysis using RNA-guided nucleases and NGS.