Rhinella arenarum transcriptome

Rhinella arenarum transcriptome provides a de novo assembled and annotated larval transcriptome of Rhinella arenarum to support gene expression, functional genomics, and phylogenetic analyses in the absence of a reference genome.

Key Features:

• Sequencing: Massive RNA sequencing of R. arenarum larvae generated the raw data.
• Assembly: De novo assembly of RNA-seq reads produced the transcript set.
• Annotation: Functional annotation and gene prediction were applied to assembled transcripts.
• Transcript inventory: The dataset comprises 57,407 well-annotated transcripts.
• Completeness: The transcriptome reports 99.4% completeness.
• lncRNA catalog: Identification of 52,800 high-confidence long non-coding RNA (lncRNA) transcripts.
• Validation: Reliability was demonstrated through application in phylogenetic analysis.

Scientific Applications:

• Gene expression analysis: Enables differential expression and transcriptional profiling in R. arenarum larvae.
• Functional genomics: Supports identification of gene functions and regulatory elements including lncRNAs.
• Phylogenetics: Enables phylogenetic and evolutionary studies using assembled transcripts.
• Ecotoxicology biomarkers: Facilitates identification of molecular biomarkers relevant to environmental toxicology and ecotoxicological research.
• Non-model organism research: Enables molecular studies in a non-model amphibian lacking a reference genome.

Methodology:

Massive RNA sequencing of R. arenarum larvae was followed by de novo assembly, annotation, gene prediction, and validation via phylogenetic analysis.