Ribocentre-Aptamer

Ribocentre-Aptamer systematically catalogues RNA aptamer data to capture the molecular mechanism of RNA aptamer binding and bridge molecular mechanism discovery with translational engineering.

Key Features:

• Manually curated database: The resource manually curates 191 aptamer types across 669 publications.
• Sequence integration: The database unifies 510 sequences with experimentally resolved structural and functional data.
• Ligand coverage: The database includes 123 small-molecule ligands and 344 protein ligands.
• Structural data: The resource provides experimentally resolved secondary and tertiary structures.
• Binding mechanism annotations: The database includes ligand-binding pockets, affinity metrics, and functional annotations.

Scientific Applications:

• Diagnostics: RNA aptamers enable transformative applications in diagnostics through binding to diverse ligands.
• Therapeutics: RNA aptamers function as 'chemical antibodies' with advantages in binding to diverse ligands for therapeutic applications.
• Biosensing: RNA aptamers enable transformative applications in biosensing through ligand binding.
• Rational aptamer design: The database addresses the lack of integrated resources coupling experimentally determined structural data with ligand-binding mechanisms to facilitate rational design.
• Molecular mechanism discovery: The resource captures the molecular mechanism of RNA aptamer binding.
• Translational engineering: The database bridges molecular mechanism discovery with translational engineering for advancing aptamer science.

Methodology:

Manual curation of literature data; unification of sequences, small-molecule ligands, and protein ligands with experimentally resolved secondary/tertiary structures, ligand-binding pockets, affinity metrics, and functional annotations.